Cytosolic drug transportation is crucial for effective treatments. Two of the major challenges are for drugs to reach the target tissues and then on a subcellular level, to escape from endosomes and reach their targeted sites in the cytosol. We tackled this problem by using amphiphilic nanoparticles (amph-NPs) as cytosolic drug carriers. These particles embed and traverse lipid membranes reversibly without membrane disruption. Amph-NP can carry small molecule drugs effectively into numerous cell lines by embed in and traverse in between intracellular membranes. We aim to enhance immunomodulatory drug delivery of immune-compromised animal models via amph-NP drug transportation.